Preface ix
Acknowledgments xi
Authors xv
About the Companion Website xvii
Chapter 1 Fecal Examination for the Diagnosis of Parasitism 1
Collection of Fecal Samples 1
Storage and Shipment of Fecal Samples 2
Fecal Exam Procedures 2
Fecal Flotation 3
Additional Procedures for Fecal Examination 12
Quality Control for Fecal Exam Procedures 15
Use of the Microscope 16
Microscope Calibration 16
Pseudoparasites and Spurious Parasites 19
Identification of Nematode Larvae Recovered with Fecal Flotation or Baermann Procedures 24
Techniques for Evaluation of Strongylid Nematodes in Grazing Animals 29
Fecal Culture 29
Identification of Ruminant and Camelid Third-Stage Larvae 30
Identification of Third-Stage Larvae of Equine Strongyles 35
Fecal Egg Count Reduction Test (FECRT) 36
Hoyer’s Solution 39
Lactophenol 40
Parasites of Domestic Animals 41
Dogs and Cats 42
Ruminants and Camelids 96
Horses 126
Swine 140
Birds 154
Rodents and Rabbits 174
Reptiles 182
Chapter 2 Detection of Protozoan and Helminth Parasites in the Urinary, Reproductive, and Integumentary Systems and in the Eye 191
Techniques for Parasite Recovery 191
Parasites of the Urinary System 191
Parasites of the Reproductive Tract 192
Helminth Parasites of the Integumentary System 192
Parasite Detection in Urinary and Other Systems 193
Chapter 3 Detection of Parasites in the Blood 207
Immunologic and Molecular Detection of Blood Parasites 207
Microscopic Examination of Blood for Protozoan Parasites 207
Giemsa Stain 208
Microscopic Examination of Blood for Nematode Parasites 209
Tests for Canine Heartworm Microfilariae in Blood Samples 210
Blood Parasites of Dogs and Cats 213
Blood Parasites of Livestock and Horses 228
Blood Parasites of Birds 234
Chapter 4 Immunodiagnostic and Molecular Diagnostic Tests in Veterinary Parasitology 239
Immunodiagnostic Methods in Parasitology 239
Molecular Diagnostic Methods in Parasitology 243
Chapter 5 Diagnosis of Arthropod Parasites 247
Subclass Acari (Mites and Ticks) 247
Mite Identification 247
Tick Identification 278
Class Insecta 300
Lice (Order Phthiraptera) 300
Fleas (Order Siphonaptera) 314
Flies (Order Diptera) 322
Other Insects 342
Chapter 6 Parasites of Fish 347
Techniques for Recovery of Ectoparasites 347
Skin Biopsy (Mucus Smear) 348
Fin Biopsy (Fin Snip) 348
Gill Biopsy (Gill Snip) 348
Recovery of Endoparasites 349
Parasites of Fish 350
Chapter 7 Treatment of Veterinary Parasites 371
Introduction 371
Anthelmintics 371
Specific Anthelmintics 372
Ectoparasiticides 375
Protozoal Treatment 378
Non-Traditional Treatments 379
Chapter 8 Diagnostic Dilemmas 381
Diagnostic Dilemma 1 381
Diagnostic Dilemma 2 382
Diagnostic Dilemma 3 383
Diagnostic Dilemma 4 383
Diagnostic Dilemma 5 384
Diagnostic Dilemma 6 385
Diagnostic Dilemma 7 386
Bibliography 387
Index 391